Phenotyping TILs in situ: The enumeration and quantitation of phenotypic subsets of immune cells in tissue sections
Presented by: James Mansfield, Director -Tissue Analysis Applications, PerkinElmer
View our on demand webinar demonstrating the use of automated methods for counting Tregs, Tacts and other immune cells in follicular lymphoma, melanoma and lymph nodes. Automated, multiplexed tissue cytometric analyses are feasible for routine clinical studies, work with many multiplexed IHC staining methodologies for a range of immune cell types and are of importance for translational cancer studies in general and cancer immunotherapy in particular.
In many cancers, tumor-infiltrating lymphocytes (TILs) indicate levels of tumor immunogenicity and predict survival. In particular, increased levels of regulatory T-cells (Tregs) are associated with poorer prognosis, while CD69+ T-cells may also be prognostic. Understanding the phenotype and pattern of TILs in situ within tumors would be advantageous. However, visual TIL assessment cannot easily determine the type of lymphocyte in situ, and multimarker quantitation is difficult with standard methods. We present a multi-marker, computer-aided event-counting method for determining the phenotypes of lymphocytes in a range of tumor types using a multispectral imaging (MSI) automated tissue segmentation and counting approach.