In this webinar, presented by Dr. Imre Gaspar of the Developmental Biology Unit at EMBL, Heidelberg, you will learn:
- Importance of mRNA localization and function of mRNPs.
- Advantages of using fluorogenic FIT probes to visualize mRNPs in vivo and in fixed specimen.
- How super-resolution microscopy can identify factors required for mRNP biogenesis.
Wednesday, Oct 28, 2015
4PM London, 12PM Boston, 9AM San Diego
All mRNA molecules recruit specific proteins to form ribonucleoprotein complexes (mRNPs) . Composition and localization of many mRNPs change dynamically from translation to decay. Microscopic techniques with high spatial and temporal resolution are invaluable for studying mRNP biogenesis.
We have developed new tools based on fluorogenic forced intercalation (FIT) probes for RNA detection, quantification and interference in biological samples. The probes contain a thiazole orange (TO) dye introduced at a position normally occupied by a nucleobase. Upon binding to target nucleic acids, the TO dye increases its quantum yield and brightness substantially (greater than10 fold). These probes detect mRNA in a rapid, wash-free FISH setup using conventional wide-field microscopy. It is an ideal tool for RNA localization screens .
1. V Marchand, I Gaspar, A Ephrussi. Curr Opin Cell Biol 2012; 24(2)
2. F Hövelmann, I Gaspar, A Ephrussi, O Seitz. J Am Chem Soc 2013; 135(50)
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